Review: Rational, evolutionary, and AI-based design of cross-species AAV capsids

A review outlines rational design, directed evolution, and AI-based methods to engineer AAV gene therapy vectors with improved cross-species translatability, addressing receptor binding, trafficking, immunity, and toxicity barriers.

• Review article; no original data.
• AI-based design is one of the three strategies discussed, alongside rational design and directed evolution.
• No new experimental validation; the challenge of cross-species translation is framed as ongoing.

RadPigeon summaries are original and for information only. They are not clinical advice.